Konstantin Severinov has co-authored a paper an article “Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages” that has been recently published in Computational and Structural Biotechnology Journal. The paper was previously released at bioRxiv. The scientists developed ONT-cappable-seq, a specialized long-read RNA sequencing technique that allows end-to-end sequencing of primary prokaryotic transcripts using the Nanopore sequencing platform. They applied ONT-cappable-seq to study transcription of Pseudomonas aeruginosa phage LUZ7, obtaining a comprehensive genome-wide map of viral transcription start sites, terminators, and complex operon structures that fine-regulate gene expression. The work provides new insights in the RNA biology of a non-model phage, unveiling distinct promoter architectures, putative small non-coding viral RNAs, and the prominent regulatory role of terminators during infection. Full text of the paper is available here.