Petr Sergiev and Olga Dontsova co-authored a paper “Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection” that was published in International Journal of Molecular Sciences. In this work, the scientists compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. These systems were designed to overcome the problem of prevalence of non-homologous end joining over homologous recombination, because only homologous recombination is suitable to introduce particularly desired genetic variants.. They observed that homologous recombination utilizing a single-strand DNA template following single guide RNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations. Full text of the paper is available here.