Natalia Nikitchina

Research advisor: Professor Konstantin Severinov

Program entry year: 2019

Graduated from IKBFU, Faculty of Bioengineering and Bioinformatics in 2019

Research project:  Use of CRISPR-Cas editors for human mitochondrial genomes editing

Research interests: Mitochondrial biology, technologies for targeted editing of nuclear and mitochondrial DNA

Academic mobility:
University: University of Strasbourg, Strasbourg, France

Laboratory: Génétique Moléculaire, Génomique, Microbiologie (GMGM), https://gmgm.unistra.fr/

Dates: 01.02.2020 – 17.08.2020; 01.02.2021 – 30.06.2021; 16.11.21 – 30.11.22 

Publications:

• Future of human mitochondrial DNA editing technologies. Verechshagina, N., Nikitchina, N., Yamada, Y., Harashima, Н., Tanaka, M., Orishchenko, K., & Mazunin, I. Mitochondrial DNA Part A. 2019 Mar;30(2). DOI: 10.1080/24701394.2018.1472773
• Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA. Shebanova, R. and Nikitchina, N., Shebanov, N., Mekler, V., Kuznedelov, K., Ulashchik, E., Vasilev, R., Sharko, O., Shmanai, V., Tarassov, I., Severinov, K., Entelis, N., Mazunin, I. 2021 Nucleic acids research, 50(2), 1162-1173. DOI: 10.1093/nar/gkab1227
• Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii. Vasilev, R., Gunitseva, N., Shebanova, ,R., Korzhenkov, A., Vlaskina, A., Evteeva, M., Polushkina, I., Nikitchina, N., Toshchakov, S., Kamenski, P., Patrushev, M., Mazunin, I.
  2022 International Journal of Molecular Sciences, 23(16), 9289. DOI: 10.3390/ijms23169289

Conference presentations:

• Modification of RGEN/AsCpf1 elements for import and function inside of the human mitochondrion. Nikitchina N, Verechshagina N, Shebanov N, Vyatkin Y, Orishchenko K, Mazunin I. CRISPR 2018, Vilnius, Lithuania, 2018 June 20-23. DOI: 10.13140/RG.2.2.35230.43844 . Poster presentation
• Use of the Type 2 CRISPR-Cas systems for human mitochondrial genomes editing. Nikitchina N, Mazunin I, Entelis N, Tarassov I. 11th World Congress on Targeting Mitochondria 2020, Berlin, Germany, 2020 October 28-30. Short oral presentation 
• Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA. Nikitchina N, Shebanova R, Shebanov N, Tarassov I, Severinov K, Entelis N, Mazunin I. CRISPR and Translational Medicine Congress 2022, Bordeaux, France, 2022 March 31 – April 01. Short oral presentation 

Grants:

•  2017-2019 – Grant from the Russian Science Foundation № 17-75-20015 “Molecular genetics technologies of targeted human mitochondrial genome editing”
• 2017-2020 – State Task Grant  “Functional assessment of the modified mitochondrial DNA which is not subject to the formation of deletions”
• 2019-2021 – RFBR mс 19-29-04101 “Studying the mechanism of control of mitochondrial replication/transcription by development and application of the mtDNA CSBII editing technology”