Ishita Jain

Research advisor: Konstantin Severinov

Program entry year: 2017

Graduated from Rutgers, The State University of New Jersey in 2016
Master of Science in Microbial Biology
Thesis project: Characterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong delta in Vietnam
Supervisor: Max M. Haggblom

Research project: Comparative Analysis of Interference and Adaptation Mechanisms in Class 2 CRISPR-Cas Systems

Research interests: Bacterial defense systems (CRISPR-Cas)

Academic mobility:
University: Rutgers, The State University of New Jersey

Laboratory: Severinov Lab, Waksman Institute of Microbiology https://www.waksman.rutgers.edu/severinov/home

Dates: 01.11.2017 – ongoing

Publications:

• Defining the seed sequence of the Cas12b CRISPR-Cas effector complex. Jain I, Minakhin L, Mekler V, Sitnik V, Rubanova N, Severinov K and Semenova E. RNA Biol. 2019 Apr;16(4):413-422. doi: 10.1080/15476286.2018.1495492

• Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation. Kuznedelov K, Mekler V, Lemak S, Tokmina-Lukaszewska M, Datsenko KA, Jain I, Savitskaya E, Mallon J, Shmakov S, Bothner B, Bailey S, Yakunin AF, Severinov K, Semenova E. Nucleic Acids Res. 2016 Dec 15; 44(22): 10849–10861. doi: 10.1093/nar/gkw914

Conference presentations:

• Decoding the mechanism of CRISPR interference by RNA-guided RNA-targeting Cas13a effector. Jain I, Kolesnik M, Minakhin L, Morozova N, Borukhov S, Semenova E, Severinov K. Cell Symposia Regulatory RNAs 2019, Berlin, Germany. Poster presentation
• An internal specificity determinant of Type V-B systems target recognition. Jain I, Minakhin L, Mekler V, Sitnik V, Rubanova N, Severinov K, Semenova E. CRISPR 2018, Vilnius, Lithuania. Poster presentation
• Laying the seed of the Type V-B CRISPR-Cas system C2c1. Jain I, Minakhin L, Mekler V, Sitnik V, Rubanova N, Datsenko KA, Severinov K, Semenova E. CRISPR 2017, Montana, USA. Poster presentation