Artem Isaev

Research advisor: Severinov Konstantin

Program entry year: 2017

Graduated from Skoltech Master Program in 2017

Research project: Study of the mechanisms of action of bacterial defense system BREX

Research interests: phage-host interactions, bacterial defense, restriction-modification

Academic mobility:
University: York University, UK

Laboratory: Microbiology Department, group of Dr. Maggie Smith

Dates: 02.2017 – 04.2017

Publications:

• Anikaev A.Y., Isaev A.B., Korobeinikova A.V., Garber M.B., Gongadze G.M. Role of protein L25 and its contact with protein L16 in maintaining the active state of Escherichia coli ribosomes in vivo. Biochemistry, Vol. 81, p. 19-27, 2016
• BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K., Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L., Severinov K. Nucleic Acids Res., Vol. 47(1), p.253-265, 2019

Conference presentations:                                                                                                                                                                                           

• Isaev A.B., Tsvetkova K. M., Pechenov P. Y., Matlashov M. E., Severinov K. V. Bacterial BREX defense system affects replication and transcription of phage genomes. 20-th School-conference of Young Scientists in Pushchino, Pushchino, 2016. Oral Presentation 
• Isaev A.B., Matlashov M. E., Tsvetkova K. M., Pechenov P. Y., Severinov K. V.  Some insights from the study of BREX – a novel phage resistance system. Bacterriophages: Theoretical and Practical Aspects of Their Application, Moscow, 2016. Oral Presentation                                                                                                                                                                                                   
• Isaev A.B., Gordeeva J.M., Tsvetkova K. M.,  Matlashov M. E., Severinov K. V. Insights from Study of BREX- Novel Bacterial Defence System. Molecular Genetics of Bacteria and Phages Meeting, Wisconsin, Madison, USA, 2018. Poster presentation
• Gordeeva J., Morozova N., Sierro N., Isaev A., Ivanov N.V., Zeng L., Severinov K.  BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. CRISPR-2018, Vilnius, Lithuania, 2018 June 20-23. Poster presentation
• Isaev A.B., Gordeeva J.M., Tsvetkova K. M.,  Matlashov M. E., Severinov K. V. Characterization of phage defence system BREX from Escherichia coli HS. Bacteriophage in Medicine, Food & Biotechnology, Oxford, UK, 2018. Oral Presentation
• Isaev A., Drobiazko A.M., Gordeeva J., Severinov K. DNA mimic protein Ocr from phage T7 is a potent inhibitor of BREX defense system. CRISPR-2019, Quebec, Canada, 2019. Poster presentation
• Isaev A., Drobiazko A.M., Gordeeva J., Severinov K. DNA mimic protein Ocr from phage T7 is a potent inhibitor of BREX defense system. 3rd International Conference on CRISPR Technologies, Wurzburg, Germany, 2019. Poster presentation

Grants:

• 2018-2020 – RFBR grant mol_a №18-34-00845  “Study of the mechanisms of invading DNA recognition by bacterial defence system BREX from Escherichia coli HS”
• 2018-2020 – UMNIK grant “Development of hybrid transducing particles for plasmid delivery into the cells of Streptomyces species”
• 2019-2021 – RFBR grant for PhD students №19-34-90160 “Study of the mechanisms of BREX system inhibition by DNA mimic protein Ocr from phage T7″