Artem Isaev

Research advisor: Severinov Konstantin

Program entry year: 2017

Graduated from Skoltech Master Program in 2017

Research project: Study of the mechanisms of action of bacterial defense system BREX

Research interests: phage-host interactions, bacterial defense, restriction-modification

Academic mobility:
2017 – York University, UK, group of Dr. Margareth Smith – Study of the Pgl defence system from Streptomyces

2019 – Toronto University, Canada, group of Dr. Karen Maxwell – Study of the phage genome injection in the presence of BREX defence system

Publications:

• Role of protein L25 and its contact with protein L16 in maintaining the active state of Escherichia coli ribosomes in vivo. Anikaev A.Y., Isaev A.B., Korobeinikova A.V., Garber M.B., Gongadze G.M.  Biochemistry (Moscow), Vol. 81, p. 19-27, 2016
• BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K., Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L., Severinov K. Nucleic Acids Research, Vol. 47(1), p.253-265, 2019
• Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence. Isaev A., Drobiazko, A., Sierro, N., Gordeeva, J., Yosef, I., Qimron, U., Ivanov N.V., Severinov, K.  Nucleic Acids Research, 48(10), 5397-5406, 2020.

Conference presentations:                                                                                                                                                                                           

• Isaev A.B., Tsvetkova K. M., Pechenov P. Y., Matlashov M. E., Severinov K. V. Bacterial BREX defense system affects replication and transcription of phage genomes. 20-th School-conference of Young Scientists in Pushchino, Pushchino, 2016. Oral Presentation 
• Isaev A.B., Matlashov M. E., Tsvetkova K. M., Pechenov P. Y., Severinov K. V.  Some insights from the study of BREX – a novel phage resistance system. Bacterriophages: Theoretical and Practical Aspects of Their Application, Moscow, 2016. Oral Presentation                               
• Isaev A.B., Gordeeva J.M., Tsvetkova K. M.,  Matlashov M. E., Severinov K. V. Insights from Study of BREX- Novel Bacterial Defence System. Molecular Genetics of Bacteria and Phages Meeting, Wisconsin, Madison, USA, 2018. Poster presentation
• Gordeeva J., Morozova N., Sierro N., Isaev A., Ivanov N.V., Zeng L., Severinov K.  BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site. CRISPR-2018, Vilnius, Lithuania, 2018 June 20-23. Poster presentation
• Isaev A.B., Gordeeva J.M., Tsvetkova K. M.,  Matlashov M. E., Severinov K. V. Characterization of phage defence system BREX from Escherichia coli HS. Bacteriophage in Medicine, Food & Biotechnology, Oxford, UK, 2018. Oral Presentation
• Isaev A., Drobiazko A.M., Gordeeva J., Severinov K. DNA mimic protein Ocr from phage T7 is a potent inhibitor of BREX defense system. CRISPR-2019, Quebec, Canada, 2019. Poster presentation
• Isaev A., Drobiazko A.M., Gordeeva J., Severinov K. DNA mimic protein Ocr from phage T7 is a potent inhibitor of BREX defense system. 3rd International Conference on CRISPR Technologies, Wurzburg, Germany, 2019. Poster presentation
• Isaev A., Drobiazko A.M., Gordeeva J., Severinov K. DNA mimic protein Ocr from phage T7 is a potent inhibitor of BREX defense system. 2020 Global Young Scientists Summit, Singapore, 2020. Poster presentation

Grants:

• 2018-2020 – RFBR grant mol_a №18-34-00845  “Study of the mechanisms of invading DNA recognition by bacterial defence system BREX from Escherichia coli HS”
• 2018-2020 – UMNIK grant “Development of hybrid transducing particles for plasmid delivery into the cells of Streptomyces species”
• 2019-2021 – RFBR grant for PhD students №19-34-90160 “Study of the mechanisms of BREX system inhibition by DNA mimic protein Ocr from phage T7″
• 2020 – RFBR grant expansion №19-14-50560 “Mechanisms of bacterial protection against viral infection”
• 2021-2022 – RFBR grant KO_a №21-54-10001 – joint project with Dr. Tim Blower from Durham University “Structural and functional studies of BREX phage-resistance systems and their phage-encoded inhibitors”