Anna Shiriaeva

Research advisor: Professor Konstantin Severinov
Program entry year: 2016
Graduated from Saint-Petersburg State University, Biological Faculty, Department of Genetics and Biotechnology in 2015
Research project: The mechanisms of adaptation in type I CRISPR-Cas systems
Research interests: CRISPR/Cas systems, DNA replication and repair
Academic mobility:
University: Waksman Institute of Microbiology, Rutgers University, USA
Laboratory: Severinov Laboratory https://www.waksman.rutgers.edu/severinov/home
Dates: June 28, 2017 – May 29, 2018; February 27, 2019 – July 21, 2019
Publications:

• Anna Shiriaeva, Ekaterina Savitskaya, Kirill Datsenko, Irina Vvedenskaya, Iana Fedorova, Natalia Morozova, Anastasia Metlitskaya, Anton Sabantsev, Bryce Nickels, Konstantin Severinov, and Ekaterina Semenova (2019). Detection of Spacer Precursors Formed In Vivo During Primed CRISPR Adaptation. Nature Communications. 10, 4603.
• Stepchenkova, E.I., Shiriaeva, A.A., and Pavlov, Y.I. (2018). Deletion of the DEF1 gene does not confer UV-immutability but frequently leads to self-diploidization in yeast Saccharomyces cerevisiae. DNA Repair (Amst.) 70, 49–54.

Conference presentations:

• Anna Shiriaeva, Ekaterina Savitskaya, Elena Kurilovich, Anastasia Metlitskaya, Kirill Datsenko, Ekaterina Semenova, Konstantin Severinov Detection of RecBCD-generated DNA products in Escherichia coli cells undergoing CRISPR interference, 3rd International Conference on CRISPR Technologies, September 16-18, 2019, Würzburg, Germany: poster presentation.
• Anna Shiriaeva Detection of Spacer Precursors Formed in vivo During Primed Adaptation, Rutgers – Wageningen Graduate Student Symposium, June 13, 2019, New Brunswick, USA: oral presentation.
• Anna Shiriaeva, Ekaterina Savitskaya, Kirill Datsenko, Irina Vvedenskaya, Bryce Nickels, Ekaterina Semenova, Konstantin Severinov In vivo detection of primed adaptation intermediates in Escherichia coli, CRISPR2018, June 20-23, Vilnius, Lithuania: poster presentation.
• Shiriaeva A., Savitskaya, Semenova E., Datsenko K.A., Severinov K. RecBC influences CRISPR Adaptation in the Type I-E CRISPR-Cas System of Escherichia coli, Waksman Annual Retreat, Rutgers University, NJ, USA, September 12, 2017: poster presentation.
• Shiriaeva A. Interplay between cellular DNA maintenance mechanisms and CRISPR-Cas machinery in Escherichia coli, 1^st Annual Workshop of Skoltech/MIT Next Generation Program, Moscow, October 25-26, 2016: oral presentation.

Grants:

•  UMNIK support of Russian Foundation for Assistance to Small Innovative Enterprises (FASIE) for the project: Development of accelerated version of genetic toxicology system “Alpha-test” (2015)
• Grant of Saint-Petersburg Administration for students and PhD students (2014)