PI – Petr Sergiev
Ph.D. Students – Ekaterina Komarova, Evgenia Zotova
Start date – 2017
Main focus of the group is identification and analysis of antibiotics acting on the ribosome. The group of P. Sergiev created a reporter strain of E.coli that contains two sensors of antibiotic action. The red fluorescent protein gene is induced upon DNA damage, caused by antibiotics, such as fluoroquinolones. The far red fluorescent protein gene Katushka2S is induced if cells are treated with inhibitors of protein biosynthesis. The system is well suited for high throughput screening of new antibiotics with the immediate recognition of the molecular target of antibiotic action. Application of this system allowed us to study mechanism of action of several antibiotics.