Supervisor: Konstantin Severinov, co-advisor – Eugene V. Koonin, NIH/NLM/NCBI
Entry year to Skoltech – 2014
Master of CS, BMSTU Baumansky Moscow State Technical University
Research project: Discovery of novel viruses, mobile elements and defence systems.
This project is closely related to CRISPR system and Cas9 genes that are now emerging field of gene editing methods.
Before Cas9 revolution procedure of making genetically modified animals took 2 years and a lot of resources. Now with Cas9 it takes few months and much less costs.
By conducting this research we may be able to find new more effective variants of Cas9 or other defence/attack system types that can be used in medicine.
“Effective decision making program complex based on fuzzy controllers”
BMSTU 2008, supervisor: Rogozin EV
Science. 2016 Aug 5;353(6299):aaf5573. doi: 10.1126/science.aaf5573. Epub 2016 Jun 2.
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector.
Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F.
Front Microbiol. 2016 Mar 31;7:398. doi: 10.3389/fmicb.2016.00398. eCollection 2016.
Metagenomic Analysis of Bacterial Communities of Antarctic Surface Snow.
Lopatina A, Medvedeva S, Shmakov S, Logacheva MD, Krylenkov V, Severinov K.
Genome Biol Evol. 2016 Jan 13;8(2):375-86. doi: 10.1093/gbe/evw006.
Recent Mobility of Casposons, Self-Synthesizing Transposons at the Origin of the CRISPR-Cas Immunity.
Krupovic M, Shmakov S, Makarova KS, Forterre P, Koonin EV.
Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems.
Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV.
Nucleic Acids Res. 2014 May;42(9):5907-16. doi: 10.1093/nar/gku226. Epub 2014 Apr 11.
Pervasive generation of oppositely oriented spacers during CRISPR adaptation.
Shmakov S, Savitskaya E, Semenova E, Logacheva MD, Datsenko KA, Severinov K
NIH Director award 2016: “For his work on discovery and functional classification of diverse Class 2 CRISPR-Cas systems”