aleksandrastrotskaya

Aleksandra Strotskaya

PhD student

Supervisor: Konstantin Severinov

Entry Year to Skoltech – 2013

Graduated from Moscow Academy of Veterinary Medicine and Biotechnology with diploma work on the “Isolation of minor enterobacteriophages from animal feces and their characteristics» made in Laboratory of microbial viruses, Institute of Microbiology, RAS (Research group leader Dr. A. Letarov).

Research project: The mechanisms of adaptation and interference in CRISPR/Cas system against a variety of virulent phages with different strategies of infection.

In Escherichia coli, the acquisition of new CRISPR spacers is strongly stimulated by a priming interaction between a spacer in CRISPR RNA and a protospacer in foreign DNA. This phenomenon, which we refer to as “priming”, can be used for very simple and rapid construction of multiple E. coli strains capable of targeting, through CRISPR interference, any phage or plasmid of interest. Availability of such strains should allow rapid progress in the analysis of CRISPR-Cas system function against diverse mobile genetic elements.

Publications:

Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C. Kulikov E, Kropinski AM, Golomidova A, Lingohr E, Govorun V, Serebryakova M, Prokhorov N, Letarova M, Manykin A, Strotskaya A, Letarov A. (2012) Virology. 426(2):93-9

Rapid multiplex creation of Escherichia coli strains capable of interfering with phage infection through CRISPR. Strotksaya A., Semenova E., Savitskaya E., Severinov K. (2015) Methods Mol.Biol.1311:147-159

Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex. Semenova E., Savitskaya E., Musharova O., Strotskaya A., Vorontsova D., Datsenko K.A., Logacheva M.D., Severinov K., (2016) Proc. Natl. Acad. Sci. U.S.A. 113:7626-7631.

Conferences:

Savitskaya E., Semenova E., Strotskaya A., Datsenko K., Severinov K. High throughput analysis of type I-E CRISPR system adaptation in E.coli. MSU Moscow, Russian Federation. 2013

Savitskaya E., Strotskaya A., Musharova O., Kapustin M., Severinov K.. The dynamic interaction between CRISPR/Cas system with wild type or escape targets in Esherichia coli. EMBL Heidelberg, Germany. 2013

Strotskaya A., Savitskaya E., Severinov K.. Investigation of the process of phage infection at conditions of CRISPR-Cas targeting. Institute of Microbiology, RAS, Moscow, Russian Federation. 2015

Strotskaia A., Savitskaya E., Semenova E., Morozova N., Metlytskaya A., Severinov K.. Phage infection at conditions of CRISPR-Cas targeting, Weizman institute of science, Israel. 2016

Strotskaia A., Savitskaya E., Morozova N., Severinov K.. Bioinformatic analysis of phage infection at conditions of CRISPR-Cas targeting, ITaS, Saint-Petersburg, Russian Federation. 2016

konstantinseverinov
Konstantin Severinov Professor, Director of the Center for Data-Intensive Biomedicine and Biotechnology
mariakolesnikova
Maria Kolesnikova Assistant Director for Development
dmitripapatsenko
Dmitri Papatsenko Assistant Professor
dmitripervouchine
Dmitri Pervouchine Assistant Professor
dmitrychudakov
Dmitry Chudakov Associate Professor
georgiibazykin
Georgii Bazykin Assistant Professor
mikhailgelfand
Mikhail Gelfand Professor
petrsergiev
Petr Sergiev Associate Professor
philippkhaitovich
vladimirzelman
Vladimir Zelman Professor
annavanyushkina
Anna Vanyushkina Research Scientist
asiyaayupova
Asiya Ayupova Junior Research Scientist
daryatsibulskaya
Darya Tsibulskaya Research Intern
denisgoruynov
Denis Goryunov Research Scientist
dmitriitravin
Dmitrii Travin Research Intern
dmitryshcherbinin
Dmitry Shcherbinin Research Scientist
dmitrysutormin
Dmitry Sutormin Research Intern
dmitrysvetlichnyy
Dmitry Svetlichnyy Research Scientist
ekaterinakhrameeva
Ekaterina Khrameeva Research Scientist
ekaterinasavitskaya
Ekaterina Savitskaya Senior Research Scientist
evgenyklimuk
Evgeny Klimuk Research Scientist
liqian
Qian Li Junior Research Scientist
maratkazanov
Marat Kazanov Senior Research Scientist
mariapoliakova
Maria Poliakova Research Scientist
mariatutukina
Maria Tutukina Research Scientist
mariiaandrianova
Maria Andrianova Junior Research Scientist
mikhailshugay
Mikhail Shugay Senior Research Scientist
nadezhdaterekhanova
Nadezhda Terekhanova Junior Research Scientist
nataliamorozova
Natalia Morozova Research Intern
olgavakhrusheva
Olga Vakhrusheva Junior Research Scientist
pavelmazin
Pavel Mazin Junior Research Scientist
sofiagarushyants
Sofya Garushyants Junior Research Scientist
Svetlana Goryunova Senior Research Scientist
vasilysitnik
Vasily Sitnik Research Scientist
victormamontov
Victor Mamontov Research Intern
waltraudmair
Waltraud Mair Research Scientist
zhisonghe
Zhisong He Research Scientist
elenanabieva
Elena Nabieva Research Scientist
denisomelchenko
Denis Omelchenko Research Scientist
andreyayginin
Andrey Ayginin Research Intern
evgeniygerasimov
Evgeniy Gerasimov Research Intern
Artem Kasianov Research Scientist
viktoriashtratnikova
Viktoria Shtratnikova Research Scientist
annasperanskaya
Anna Speranskaya Senior Research Scientist
ianafedorova
Iana Fedorova Junior Research Scientist
alinaegorova
Alina Egorova Research Intern
Ekaterina Yushina Research Intern
viktoriyafedorova
Viktoriya Fedorova Research Scientist
marinaserebryakova
Marina Serebryakova Senior Research Scientist
alexeykulikovsky
Alexey Kulikovsky Laboratory Engineer
ekaterinaabramova
Ekaterina Abramova Team Assistant
ninapopova
Nina Popova Biotechnology Educational Programs Coordinator
Svetlana Dubiley Head of Biomedical Teaching Laboratory
ekaterinakorovina
Ekaterina Korovina Research and Education Program Manager
aleksandrastrotskaya
alekseimikhalchenko
alexandermartynov
Alexander Martynov PhD student
alexandertyshkovskii
annamaikova
Anna Maikova PhD student
annapavlova
Anna Pavlova PhD student
annashmelkova
Anna Shmelkova PhD student
dariavorontsova
Daria Vorontsova PhD student
dmitryzubkov
Dmitry Zubkov PhD student
iliakurochkin
Ilia Kurochkin PhD student
juliapiskunova
Julia Piskunova PhD student
kseniatsvetkova
Ksenia Tsvetkova PhD student
lanafedorova
Iana Fedorova PhD student
mariasokolova
Maria Sokolova PhD student
olgamokina
Olga Mokina PhD student
olgamusharova
Olga Musharova PhD student
sergeyshmakov
Sergey Shmakov PhD student
sofyamedvedeva
Sofya Medvedeva PhD student
tatianazyubko
Tatiana Zyubko PhD student
vitastepanova
Vita Stepanova PhD student
yuliazhitnyuk
Yulia Zhitnyuk PhD student
andreykrivoy
Andrey Krivoy PhD student
annashiriaeva
Anna Shiriaeva PhD student
brendanwilcox
Brendan Wilcox PhD student
nataliarubanova
Natalia Rubanova PhD student
valentinaburskaia
Valentina Burskaia PhD student
Alexander Tashkeev MSc student
anastasiagrigoreva
Anastasia Grigoreva MSc student
Anton Krotov MSc student
Artem Baranovski MSc student
Artem Mikelov MSc student
Daria Romanovskaya MSc student
dariatitova
Daria Titova MSc student
dariatreskova
Daria Treskova MSc student
Ekaterina Serova MSc student
ekaterinazhigalova
Ekaterina Zhigalova MSc student
elenafomenko
Elena Fomenko MSc student
Evgeniia Alekseeva MSc student
irinautkina
Irina Utkina MSc student
ivanvaliev
Ivan Valiev MSc student
Laura Korobkova MSc student
olgatolicheva
Olga Tolicheva MSc student
Sofya Panafidina MSc student
ulianamoskvina
Uliana Moskvina MSc student
Varvara Verkhova MSc student
vasilisapolinovskaya
annabelousova
Anna Belousova MSc student
eldarabdullaev
Eldar Abdullaev MSc student
elvirakinzina
Elvira Kinzina MSc student
kseniaershova
Ksenia Ershova MSc student
nvolovich
Nadezda Volovich MSc student
pshichkova
Polina Shichkova MSc student
vasilyzubov
Vasily Zubov MSc student